Proliferative activity, apoptosis and expression of oestrogen receptor and Bcl-2 oncoprotein in canine mammary gland tumours.
Resource
Journal of Comparative Pathology (SCI),134(1),74-83.
Journal
Journal of Comparative Pathology (SCI)
Journal Volume
134
Journal Issue
1
Pages
74-83
Date Issued
2006-01
Date
2006-01
Author(s)
Yang, W.Y.
Liu, C.H.
Chang, C.J.
Lee, C.C.
Chang, K.J.
Abstract
Samples of 39 canine mammary gland tumours (MGTs) were examined immunohistochemically for oestrogen receptor (ER-α), Bcl-2 protein and Ki67 antigen, and by TUNEL assay for apoptosis. ER-α was expressed by 80% (31/39) of the tumours, including all of the 15 benign tumours and 67% (16/42) of the malignant tumours. ER-α expression was greater in the benign than in the malignant tumours (P<0.01). Bcl-2 protein was detected in 62% (24/39) of the MGTs, of which 67% (10/15) were benign and 58% (14/24) malignant. No significant difference in Bcl-2 expression between benign and malignant tumours was detected. The Ki67 and TUNEL indices were greater in malignant than in benign tumours (P<0.01). Correlation analysis suggested that ER-α and Bcl-2 expression were related, but this observation lacked statistical significance. The levels of cell proliferation and apoptosis did not appear to be significantly correlated with the expression of Bcl-2. A positive relationship was apparent between cell proliferation and apoptosis, whilst a negative correlation between ER-α and cell proliferation was demonstrated. In conclusion, the suggestion of a positive correlation between ER-α and Bcl-2 in canine MGTs indicates that ER may be the regulator of Bcl-2 protein, as in human breast cancer. In contrast to cell proliferation and apoptosis, ER-α and Bcl-2 expression were greater in benign MGTs than in their malignant counterparts. ? 2005 Elsevier Ltd. All rights reserved.
SDGs
Other Subjects
estrogen receptor alpha; Ki 67 antigen; protein bcl 2; estrogen receptor alpha; Ki 67 antigen; protein bcl 2; animal experiment; animal model; animal tissue; apoptosis; article; breast carcinogenesis; breast carcinoma; breast tumor; cancer growth; cell proliferation; controlled study; dog; female; histopathology; immunohistochemistry; nick end labeling; nonhuman; protein analysis; protein expression; animal; animal disease; carcinoma; dog disease; experimental neoplasm; metabolism; udder; uterus; Animalia; Canis familiaris; Animals; Apoptosis; Carcinoma; Cell Proliferation; Dog Diseases; Dogs; Estrogen Receptor alpha; Female; Immunohistochemistry; In Situ Nick-End Labeling; Ki-67 Antigen; Mammary Glands, Animal; Mammary Neoplasms, Animal; Proto-Oncogene Proteins c-bcl-2; Uterus
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