Utilizing two-photon fluorescence and second harmonic generation microscopy to study human bone marrow mesenchymal stem cell morphogenesis in Chitosan scaffold
Date Issued
2007
Date
2007
Author(s)
Huang, Chi-Hsiu
DOI
zh-TW
Abstract
Multiphoton microscopy ,using longer wavelength of near infrared light as excitation light source has deeper penetration and less photodamage. Besides,it can capable all visible autofluorescence and second harmonic generation,making us observe specimens through autofluorescence and second harmonic generation directly without slicing and dying. Hence,I hope to apply this technology of nonlinear optical microscopy to observe and analysize in tissue engineering.
One important goal in tissue engineering is cultiviating cartilage tissue for transplantation.The most common method to cultivate cartilage is to seed human bone marrow mesenchymal stem cells on three dimensional biocompatiable and biodegradable scaffold materials,then add the signal factor TGF-β3 to induce the cells to produce collagen that would grow into cartilage tissue.In this study,we seeded mesenchymal stem cells on a chitosan scaffold and added TGF-β3 to induce cartilage tissue producing and image the process using two-photon fluorescence and second harmonic generation microscopy.
In the course of imaging,we found that the production of collagen by the addition of TGF-β3 can be followed over a four-week period. The growth of collagen is not linear with time,and it approaches saturation at around 21th day.And the collagen fibers have a tendency to grow following the shape of scaffold pores.
Subjects
雙光子
二倍頻
組織工程
間葉幹細胞
幾丁聚醣
Two photon
Second harmonic generation
Tissue Engineering
Mesenchymal stem cell
Chitosan
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-96-R94222052-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):5fa0848cd8e600f99f3f73fed254b25b