Invastigating on the Regulation of Nervous System to Cardiac Tube Beating Rate in Drosophila
Date Issued
2015
Date
2015
Author(s)
Cheng, Ya-Chen
Abstract
Cardiac automatism is critical for maintaining normal physiological function in animals. Several cardiac diseases, such as palpitations and atrial fibrillation, are caused by impairment of this process. Moreover, it has demonstrated that nervous system regulates cardiac automatism in human. However, the underlying mechanism of this regulation is unclear. The transparent surface of Drosophila at 0-1 hr APF (After Puparium Formation) makes it easy to directly examine the heart beating. The tubular-shaped dorsal vessel represents heart; therefore, it is plausible and suitable for us to identify how neurons regulate cardiac automatism. To examine the influence of nervous system on heart beating, we focused on the heart rate difference between 25°C and 29°C in Drosophila at 0-1 hr APF. We took advantage of temperature-sensitive (shits, dTrpA1) or light-sensitive (ChR2, NpHR) genetic tools to acutely and specifically activate or inhibit target neurons in Drosophila by GAL4/UAS system. We found that activation of pan-neuron by dTrpA1 at 0-1 hr APF significantly accelerated the heart rate. On the contrary, inhibition of pan-neuron by shibire temperature-sensitive mutant slowed down the heart rate difference. Activating cholinergic neurons did not alter the heart rate difference, but inhibiting cholinergic neurons decreased the alteration of heart rate difference. However, activation or inhibition of glutamatergic neurons did not have effect on heart rate. Also, we focused on the heart rhythm. We found that when inhibiting pan-neuron enhaced the rate of slower HR and decreased the rate of faster HR. Otherwise, we found that when inhibiting cholinergic neurons caused abnormal heart rhythm, but activating cholinergic neurons did not alter heart rhythm. Moreover, inhibition of glutamatergic neurons decreased pause phenomenons, but activation of glutamatergic neurons did not work on heart rhythm. Together, these results suggested some neurons exist in pan-neuron may regulate heart beating rate. And cholinergic neurons may play role in regulating heart rate and regular heart rhythm in Drosophila at 0-1 hr APF. The function of glutamatergic neurons is to maintain the heart beat in Drosophila at 0-1 hr APF 0hr.
Subjects
Drosophila
Cardiac tube
Nervous system
Heart rate
Cholinergic neurons
SDGs
Type
thesis
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