Bioremediation of n-tetradecane by a pure strain(Rhodococcus erythropolis NTU1) and a mixed culture (TN-4)
Date Issued
2005
Date
2005
Author(s)
Ho, Chung-Lin
DOI
zh-TW
Abstract
This research mainly investigated the ability of a mixed culture (TN-4) and a pure strain (NTU1) to degrade n-tetradecane under different substrate’s concentration and different medium initial acidity. Besides, this research also investigated the biodegradation of different fatty acids by utilizing NTU1 and also the analyzed of acidic organic products produced during the alkane remediation process.
Experimental results indicated that increasing concentration of tetradecane increased the tetradecane biodegradation ability of NTU1 and TN-4. However, under condition of high tetradecane concentration (2000ppmv, 3000ppmv), the ability of total alkane removal was almost similar.It may be due to the rather acidic environment (pH4), inhibiting both NTU1 and TN-4’s degradation.While the initial acidity of medium was changed to a more alkaline environment, both NTU1 and TN-4 were able to attain a better growth and completely remove the alkane. In addition, it is found that NTU1 and TN-4 attain similar tendencies for their growth and alkane biodegradability, which proves that NTU1 plays a significant role among the three strains of TN-4 .
Similar aggregation behavior was observed for NTU1 grown on both tetradecane and fatty acids. Thus, NTU1 perhaps degrades tetradecane via diterminal oxidation pathway, leading to the production of succinic acid, that were found in the metabolites by HPLC analysis.
Subjects
正十四烷
生物降解
n-tetradecane
Biodegradation
Type
thesis
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