酵母菌減數分裂檢控機制之研究
Date Issued
2006
Date
2006
Author(s)
DOI
932311B002028
Abstract
Meiosis is essential for sexual reproduction. Single round of DNA replication with two
successive nuclear divisions produce haploid gametes; and therefore, organisms maintain a
constant genetic content from generation to generation after fertilization of two gametes. To
ensure the success of meiosis, meiotic checkpoints operate to coordinate the proper order of
meiotic events. In particular, the pachytene checkpoint prevents exit from the pachytene stage of
meiotic prophase when meiotic recombination and chromosome synapsis are incomplete. Our
research is planned to study the molecular mechanism of the pachytene checkpoint in detail.
In budding yeast Saccharomyces cerevisiae, the NDT80 gene encodes a meiosis-specific
transcription activator that is required for progression from pachytene into meiosis I. We have
proposed that Ndt80 is a direct target of the pachytene checkpoint. In our previous studies on
defining Ndt80 functional domains, we have isolated a dominant deletion mutation, NDT80-bc.
This Ndt80-bc protein is resistant to the control of the pachytene checkpoint and becomes
constitutively active. The NDT80-bc mutant provides a strong evidence for our hypothesis and a
powerful tool for the studies on the mechanism of the pachytene checkpoint.
This research project is to study the molecular interaction between Ndt80-bc and the pachytene
checkpoint. The Ndt80-interacting protein of the pachytene checkpoint machinery will be
isolated. Our data showed that Ndt80 was retained in cytoplasm in the zip1 mutant, while
Ndt80-bc was localized into the nucleus of the same cell. These results suggest that nuclear
localization of Ndt80 is regulated by the pachytene checkpoint. In the pachytene-arrest cells, the
Ndt80 protein is retained in the cytoplasm by an inhibitor interacting with the bc target domain
of Ndt80.The information learned from this project will provide valuable information for the
understanding of meiotic cell-cycle control. It is also useful and applicable to researches on
mitotic cell cycle, human genetic diseases, and cancers.
Subjects
meiosis
cell cycle
pachytene checkpoint
budding yeast
SDGs
Publisher
臺北市：國立臺灣大學分子與細胞生物學研究所
Type
report
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