Metallic tip enhanced fluorescence for DNA replication monitoring.
Journal
35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013, Osaka, Japan, July 3-7, 2013
Pages
488-491
Date Issued
2013
Author(s)
Abstract
We have successfully performed localized loop-mediated isothermal reactions of hepatitis B virus (HBV) and hepatitis C virus (HCV) on the apex (50?100 nm) of metallic tips coated with Bst polymerases. The SYBR green molecules binding to the new formed HBV DNA inside the optical near fields were excited by two-photon fluorescence microscopy, and directly imaged in far field. Another reporter primer is used for HCV replication detection. Preliminary results are presented in this manuscript. ? 2013 IEEE.
SDGs
Other Subjects
DNA replications; Enhanced fluorescence; Hepatitis B virus; Hepatitis C virus; Isothermal reactions; Optical near field; Replication detections; Two-photon fluorescence microscopy; Viruses; Facsimile; DNA directed DNA polymerase; immobilized enzyme; primer DNA; virus DNA; chemistry; devices; DNA replication; fluorescence; genetics; Hepacivirus; Hepatitis B virus; human; kinetics; molecular diagnosis; multiphoton microscopy; nucleic acid amplification; DNA Primers; DNA Replication; DNA, Viral; DNA-Directed DNA Polymerase; Enzymes, Immobilized; Fluorescence; Hepacivirus; Hepatitis B virus; Humans; Kinetics; Microscopy, Fluorescence, Multiphoton; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques
Type
conference paper