登革病毒血清型專一性抗體的B細胞抗原決定位及評估其為偵檢試劑之可行性
Date Issued
2002
Date
2002
Author(s)
吳漢忠
DOI
902320B002208
Abstract
Viral infection usually results in the
production of antibodies directed against the
epitopes of viral proteins through the
activation of the host’s humoral immunity.
Identification of these epitopes on viral
proteins is important for understanding the
pathogenesis of viral infectious diseases as
well as for developing vaccines and
diagnostic reagents. Serotype-specific
monoclonal antibody, Ab4, against dengue
virus type 2 (DEN-2) was generated and
identified by ELISA, immunofluorescence
and immunoblotting analysis. The B-cell
epitope of Ab4 was further identified from a
random peptide library displayed on phage.
The selected phage clones were specifically
reacted with Ab4 and did not reacted with
other monoclonal antibodies. These
immunopositive phage clones displayed a
consensus motif, His-Arg-Leu/Ile. Synthetic
peptide corresponding to the phage-borne
peptide can bind the antibody specifically.
The epitope-based synthetic peptide could
detect serum samples from DEN-2
immunized mice and rabbits by ELISA assay.
The peptide was also able to differential very
clearly between serum samples from DEN-2
and Japanese encephalitis virus (JEV)
immunized mice. The results show that this
monoclonal antibody and its epitope-based
peptide will be useful for serologic diagnosis
of DEN-2. Furthermore, the identification of
the epitope of DEN-2 will permit us to
functionally dissect the antibody response
and to address the role of antibody in the
pathogenesis of primary or secondary DEN-2
infection.
Subjects
monoclonal antibody
B-cell
epitope
epitope
synthetic peptide
serologic
diagnosis
diagnosis
SDGs
Publisher
臺北市：國立臺灣大學醫學院口腔生物科學研究所
Type
journal article
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