Secretome from Magnetically Stimulated Muscle Exhibits Anticancer Potency: Novel Preconditioning Methodology Highlighting HTRA1 Action
Journal
Cells
Journal Volume
13
Journal Issue
5
Date Issued
2024-03-05
Author(s)
Tai, Yee Kit
Iversen, Jan Nikolas
Chan, Karen Ka Wing
Fong, Charlene Hui Hua
Abdul Razar, Rafhanah Banu
Ramanan, Sharanya
Yap, Lye Yee Jasmine
Yin, Jocelyn Naixin
Toh, Shi Jie
Wong, Craig Jun Kit
Koh, Pei Fern Angele
Franco-Obregón, Alfredo
Abstract
Briefly (10 min) exposing C2C12 myotubes to low amplitude (1.5 mT) pulsed electromagnetic fields (PEMFs) generated a conditioned media (pCM) that was capable of mitigating breast cancer cell growth, migration, and invasiveness in vitro, whereas the conditioned media harvested from unexposed myotubes, representing constitutively released secretome (cCM), was less effective. Administering pCM to breast cancer microtumors engrafted onto the chorioallantoic membrane of chicken eggs reduced tumor volume and vascularity. Blood serum collected from PEMF-exposed or exercised mice allayed breast cancer cell growth, migration, and invasiveness. A secretome preconditioning methodology is presented that accentuates the graded anticancer potencies of both the cCM and pCM harvested from myotubes, demonstrating an adaptive response to pCM administered during early myogenesis that emulated secretome-based exercise adaptations observed in vivo. HTRA1 was shown to be upregulated in pCM and was demonstrated to be necessary and sufficient for the anticancer potency of the pCM; recombinant HTRA1 added to basal media recapitulated the anticancer effects of pCM and antibody-based absorption of HTRA1 from pCM precluded its anticancer effects. Brief and non-invasive PEMF stimulation may represent a method to commandeer the secretome response of muscle, both in vitro and in vivo, for clinical exploitation in breast and other cancers.
Subjects
PEMFs; aminoglycoside antibiotics; breast cancer; conditioned media; exercise; exerkines; metastasis; myokines; proteomics; soluble biomarkers
SDGs
Type
journal article