Utilizing two-photon fluorescence and second harmonic generation microscopy to study the effect of force in stem cell based chondrogensis
Date Issued
2007
Date
2007
Author(s)
Chang, Chia-Cheng
DOI
zh-TW
Abstract
Rheumatoid arthritis (RA) is a major disease for human. For a sportsman, longtime motion makes articular cartilage hurt easily. Initially we take some cartilage from other part in body to repair the hurt part. Because the more cartilage needed, we try to make the cartilage from cells. This is a big term in tissue engineering. Between all the cell’s sources, we pay much attention to use the stem cells with proper scaffolds and transfer growth factors to cultivate cartilage.
In my experiment, I utilize the auto-fluorescence and second harmonic generation to observe the forced chondrocytes producing collagen.We cultivate mesenchymal stem cells (MSCs) in a medium with TGF-β3 added. TGF-β3 induces the MSCs to differentiate into chondrocytes and the chondrocytes subsequently produce collagen. During culture, we impart force on the cells by using a centrifuge. We choose three force-0G,10000G,200000G and exerted the force on the cells for 15 minutes a day for 4 weeks. We observed that the cells exerted with greater force produce collagen that tend to accumulate in lumps at early stage, and generate stronger second harmonic signal.
Subjects
間葉幹細胞
軟骨母細胞
膠原蛋白
雙光子顯微術
二倍頻
MSCs
chondrocyte
collagen
two-photon microscopy
second harmonic generation microscopy
SDGs
Type
thesis
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