Effects of neuroprotective agents in the retinal ischemic injury model in rats
Date Issued
2006
Date
2006
Author(s)
Jeng, Yung-Yue
DOI
zh-TW
Abstract
The purpose of this study was to investigate the effects of two neuroprotective agents: Brimonidine and Phenylbutyrate (PBA), in retinal ischemic injury of rats. In adult Sprague-Dawley rats, RGCs were retrograde labeled with fluorescent tracers Fluorogold (FG) applied to both superior collicoli. Seven days later, the high intraocular pressure was induced. Medications were divided into three groups: one hour prior to retinal ischemia, 0.2% Brimonidine was instilled on the left eye; 30 minutes prior to retinal ischemia, 100 mg/kg or 400 mg/kg PBA were injected intraperitoneally; one hour after retinal ischemia, 400 mg/kg PBA was injected intraperitoneally. Rats were processed 7 days later and densities of surviving RGCs were estimated by counting FG-labeled RGCs in 16 standard regions of each retina. On the other hand, histological outcomes were also determined at 7 days after retinal ischemia. 120 mm Hg for 45 minutes ischemic injury caused 18.7% loss of FG-labeled RGCs and after the treatment of Brimonidine only 6.74% of FG-labeled RGCs were lost. Almost 100% RGCs could survive by pre-ischemic treatments of PBA but post-ischemic treatment of PBA cannot rescue RGCs from IR injury. Pre-ischemic treatment with Brimonidine and pre-ischemic treatment of PBA significantly reduced loss of thickness of retina, inner retina, and inner plexiform layer and reduced loss of cells in the ganglion cell layer and inner nuclear layer. Although post-ischemic treatment of PBA cannot rescue cells in ganglion cell layer but can reduce loss of thickness of retina, inner retina, and inner plexiform layer and reduced loss of cells in the inner nuclear layer. These results indicate that both Brimonidine and PBA have neuroprotective effects on retinal ischemia.
Subjects
青光眼
視網膜缺血
大鼠
4苯丁酸鈉
Glaucoma
Retinal ischemia
Rat
Sodium 4-Phenylbutyrate
Type
thesis
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