Optimization of Incorporating Prebiotics with Shell Materials for Microencapsulating Probiotics
Date Issued
2004
Date
2004
Author(s)
Chiu, Hsin-Yi
DOI
zh-TW
Abstract
The purpose of this research was to create a new probiotic microcapsule by using prebiotics and to attempt to apply modern optimization techniques to obtain optimal processing conditions and performance of the survival rate of probiotics. The prebiotics (peptides, fructooligosaccharides, or isomaltooligosaccharides) were incorporated with calcium alginate as wall materials to microencapsulate four probiotics (Lactobacillus acidophilus, L. casei, Bifidobacterium bifidum and B. longum). The proportion of prebiotics and calcium alginate was optimized using a response surface methodology (RSM) to build surface model first, sequential quadratic programming (SQP) and genetic algorithm (GA) were consequently used to optimize the model to evaluate the survival of microencapsulated probiotics under simulated gastro-intestinal conditions and during storage.
Optimization results indicated both GA and SQP could be used to determine the optimal combinations of wall materials for probiotic microcapsules. An optimal survival rate for micro- encapsulated probiotics was obtained at the 61 function evaluations during the SQP calculation approximately, while the GA converged to the same optimal value at 1500 function evaluations. Comparing the optimization results, this study showed that SQP was more efficient than GA in finding the optimal survival rate. The final responses in a practical case provided a result that was close to the predicted values with no apparent significant difference between both sides (P>0.05). The storage results demonstrated that addition of prebiotics in the wall materials of probiotic microcapsules provided a better protection for probiotics. Raising the proportion of prebiotics in wall materials could increase the survival of microencapsulated probiotics in the simulated gastro-intestinal tract conditions. The probiotic counts still maintained 106-107 CFU/ml for one-month microcapsules treated in simulated gastro-intestinal tests.
According to above results, incorporation of prebiotics with calcium alginate as wall materials could improve the survivability of probiotics during encapsulation, simulated gastro-intestinal conditions and storage. The current study also suggested that the two-stage effort of obtaining a surface model using RSM, and optimizing this model using SQP and GA techniques has been demonstrated to represent an effective approach. The SQP and GA all produced the optimal conditions with the SQP being the most efficient.
Subjects
益菌質
微膠囊化
原生菌
最適化
optimization
probiotics
microencapsulation
prebiotics
Type
thesis
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