Myelopoietic activity of polysaccharides extracted from Angelica sinensis
Date Issued
2007
Date
2007
Author(s)
Liu, Pei-Jou
DOI
zh-TW
Abstract
Angelica sinensis (Oliv.) Diels is a well-known herb belonging to family Umbelliferae, and is traditionally used for the treatment of anemia. The aim of this study was to investigate hematopoietic effect of extracts of Angelica sinensis (AS). For in vitro studies, the hematopoietic stem cells, collected from BALB/c mice were treated with AS, to observe the colonies-forming activity. For in vivo studies, acutely bleed BALB/c mice were administered polysaccharides which were precipitated with EtOH (ASPS) orally, to assess the hematopoietic activity of Angelica sinensis extracts.
The AS extract was subjected to membrane filtration using hollow fiber membrane module with molecular weight cut-off of 300 Da. Spleen cells from mice was treated with the permeate containing components of AS with molecular weight less than 300 Da (ASsp) and the AS polysaccharides which were precipitated with EtOH from the retentate (ASPS). Then, the spleen condition medium (ASsp-SCM, ASPS-SCM) was collected. It was found that the ASsp-SCM could not promote colony-forming in bone marrow cells. However, bone marrow cells treated with ASPS-SCM showed significant (p < 0.05) increase in colony formation as compared to AS-SCM and ASsp-SCM. Morphologic analysis of the colonies formed by ASPS-SCM showed three cell types, including granulocytes, granulocytes /monocytes and granulocytes /macrophages. The result of in vivo study showed that, the low dose of ASPS significantly (p < 0.05) increased the production of hemoglobin, while the high dose of ASPS significantly (p < 0.05) increased the levels of GM-SCF and IL-6 as compare with control and low dosage. Also it was observed that the high dose of ASPS significantly (p < 0.05) proliferated spleen cells.
This herbal medicine activates immature blood cells, possibly by suppressing cytokine secretion, thereby promoting hematopoiesis. Thus the in vitro and in vivo studies have proved that Angelica sinensis has significant hematopoietic and proliferative activity and can be used in anemia treatment.
Subjects
當歸粗多醣 (ASPS)
脾臟細胞條件培養液
造血功能
集落數
急性失血
細胞激素
Angelica sinensis polysaccharide (ASPS)
spleen cell-conditioned medium (SCM)
hematopoietic activity
colony-forming assay
acutely bleed
cytokine.
Type
thesis
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