Purification and Bioactivity of a Novel Immunomodulatory Protein from Pleurotus citrinopoleatus
Date Issued
2010
Date
2010
Author(s)
Chen, Jian-Nan
Abstract
Mushrooms have become popular sources of natural antitumor, antiviral, antibacterial, antioxidative, and immunomodulatory agents. Golden oyster mushroom, Pleurotus citrinopileatus, is a common mushroom in oriental countries for human consumption. In this study, we isolated a non-lectin functional protein (PCP-3A) from the fresh fruiting body of this mushroom and investigated its immunomodulatory and antitumor activities. Electrophoresis demonstrated that PCP-3A is a glycoprotein composed of 10 subunits, each approximately 45.0 kDa in size. In vitro cell study showed that PCP-3A inhibits the proliferation of human tumor cell line U937 in a time-dependent manner. To elucidate the mechanism of growth inhibition, flow cytometry revealed that it is capable of inhibiting the growth of U937 cells by way of S phase arrest and apoptotic induction. In addition, evaluation of the immunomodulatory effect showed that PCP-3A stimulated the secretion of cytokines TNF-α, INF-γ, IL-2, and IL-10 from human peripheral blood mononuclear cells. The anti-inflammatory of PCP-3A was also evaluated. PCP-3A was found to effectively inhibit NO generation on LPS-Induced RAW 264.7 cells. To elucidate the mechanism of NO reduction, the effects of PCP3-A on LPS-induced iNOS and COX-2 expression in RAW 264.7 cells were examined.There is positive correlation between the inhibition on the generation of NO and the expression of inducible nitric oxide synthase in macrophage.
Subjects
antitumor
anti-inflammatory
immunomodulatory
mushroom
glycoprotein
Type
thesis
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