Highly efficient inhibition of human immunodeficiency virus type 1 reverse transcriptase by aptamers functionalized gold nanoparticles
Journal
Nanoscale
Journal Volume
5
Journal Issue
7
Pages
2756
Date Issued
2013
Author(s)
Abstract
We have developed aptamer (Apt)-conjugated gold nanoparticles (Apt-Au NPs, 13 nm in diameter) as highly effective inhibitors for human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). Two Apts, RT1t49 (Apt pol) and ODN 93 (AptRH), which recognize the polymerase and RNase H regions of HIV-1 RT, are used to conjugate Au NPs to prepare Aptpol-Au NPs and AptRH-Au NPs, respectively. In addition to DNA sequence, the surface density of the aptamers on Au NPs (nApt-Au NPs; n is the number of aptamer molecules on each Au NP) and the linker length number (Tm; m is the base number of the deoxythymidine linker) between the aptamer and Au NPs play important roles in determining their inhibition activity. A HIV-lentiviral vector-based antiviral assay has been applied to determine the inhibitory effect of aptamers or Apt-Au NPs on the early stages of their replication cycle. The nuclease-stable G-quadruplex structure of 40AptRH-T45-Au NPs shows inhibitory efficiency in the retroviral replication cycle with a decreasing infectivity (40.2%). ? The Royal Society of Chemistry 2013.
SDGs
Other Subjects
Aptamers; Functionalized gold nanoparticles; G-quadruplex structure; Gold Nanoparticles; Human immunodeficiency virus type 1 reverse transcriptase; Inhibition activity; Inhibitory effect; Surface density; Diseases; Gold; Metal nanoparticles; Viruses; Gold alloys; antivirus agent; aptamer; gold; metal nanoparticle; nanoconjugate; reverse transcriptase, Human immunodeficiency virus 1; RNA directed DNA polymerase; article; cell strain HEK293; cell strain HepG2; chemistry; drug antagonism; enzymology; genetics; human; Human immunodeficiency virus 1; synthesis; Antiviral Agents; Aptamers, Nucleotide; Gold; HEK293 Cells; Hep G2 Cells; HIV Reverse Transcriptase; HIV-1; Humans; Metal Nanoparticles; Nanoconjugates
Type
journal article