行政院國家科學委員會專題研究計畫成果報告:維生素 E 藉由調控巨噬細胞功能而促進 TH1 型免疫反應
Date Issued
2000
Date
2000
Author(s)
林大盛
DOI
892313B002078
Abstract
Vitamin E (VE) is the major lipid soluble
membrane antioxidant which protects the cell
from the damage by free radicals. VE can
influence intracellular activity, though these
mechanisms are not clear. In addition, VE
can protect and stimulate cultured
lymphocytes and this is not simply due to the
decrease of membrane lipid peroxidation. It
is evident that VE can act as an
immunomodulator which is essential in
maintaining normal immunological activity.
The investigations on aging show that Th1-
type immune response is dominant when one
is young and the immune response is
switching to Th2 when one is getting old.
Because VE has anti-aging effect and also
because VE can induce IL-2 and IFN-g
production, we conclude that anti-aging
effect of VE is due to it’s enhancement of
Th1-type immune response. Thus, VE can
be an immunomodulator for Th1-type
immune response whose effects are due to
the release of cytokines: like IL-2, IFN-g by
Th1 cells. The NSC project last year
studied the modulation of lymphocytes by
VE. The preliminary results support VE as
a lymphocyte stimulator. However, other
evidences indicate that the responsiveness of
lymphocytes to mitogens and VE is enhanced
when lymphocytes are cultured with VEpretreated
macrophages. Therefore, VE
may stimulate macrophages to release
monokines like IL-1 influencing lymphocyte
response. Also, it is known that
macrophages can release IL-12 which
promotes Th1-type immune response.
Therefore, it is hypothesized that in addition
to direct lymphocyte modulation, VE can
modulate macrophages influencing their
functions which guides lymphocyte towards
Th1 immune response. The results showed
that in the presence of LPS, VE could
enhance sarcoma cell-killing ability of
macrophages. Also, macrophages, after
treatment with VE and LPS, could enhance
the proliferation of co-cultured lymphocytes.
However, macrophage IL-1 secretion was not
proved to be enhanced by ELISA, and
lymphocyte responses were not dominated by
Th1-type cytokines as measured by
ELISPOT.
Subjects
vitamin E
immunomodulation
macrophage
cytokine
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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