DPY-24蛋白質層次在Distal Tip Cells遷移中的調控機制
Regulation of DPY-24 Level in Distal Tip Cells During Gonadogenesis of C. elegans
Date Issued
2007
Date
2007
Author(s)
Ho, Guan-Wei
DOI
zh-TW
Abstract
During hermaphrodite development two distal tip cells (DTCs) migrate to generate U-shaped gonadal arms. These cells undergo three phases of migration, they first move along the ventral side to the ends of the body (phase I), then make a dorsal turn (phase II) and finally migrate back to the center of body along dorsal side (phase III). The dpy-24 gene encodes a protein with a PR domain and zinc-finger and likely controls the timing of DTC dorsal migration by transcriptional repression of unc-5. Mutations in dpy-24 resulted in the precocious dorsal turn of DTCs. Immunostaining using anti-DPY-24 antibodies detected DPY-24 protein in DTCs during migration phase I but not II or III. Interestingly, the constitutive expression of dpy-24 partially blocked DTC dorsal turn. These results together indicate that the drop of DPY-24 level is crucial for switching the migration phase of DTCs from I to II. To investigate the cause for the decrease of DPY-24 level in migration phase II, we generated various transcriptional and translational gfp reporter gene fusions. The gfp reporter construct Pdpy-24::gfp::unc-54 3’UTR carrying the dpy-24 promoter and unc-54 3’ un-translational region (UTR) and the plasmid Plag-2::gfp::dpy-24 3’UTR containing the lag-2 promoter and dpy-24 3’ UTR showed GFP expression in all migration phases I, II and III. These results indicate that the drop of DPY-24 level is likely not regulated at the transcriptional level. We then test if the regulation may be at the post-transcriptional, translational or post-translational level. We inject the construct Plag-2::dpy-24(cDNA)::unc-54 3’UTR into N2, and the results show that most of these transgenic animals are wild-type. Therefore, the regulation level of DPY-24 in DTCs is translational level, post-translational level or protein stability level.
Subjects
線蟲
生殖腺
發育
基因調控
distal tip cell
gonadogenesis
development
gene regulation
protein stability
Type
other
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