ABRC gene expression regulated by low temperature

This project was conducted to
explore the regulation and expression of
ABA response complex1 (ABRC1)
which was cloned from the promoter
region of barley HVA22 gene. Four
plasmids were used in this study which
contain 1-4 copies of ABRC1, each was
followed by Amy64 minimal promoter,
and GUS reporter gene. From PCR and
Southern blot, it revealed that 3 and 2
independent transformed cell lines
harboring 3 and 4 copies of ABRC1,
respectively, were available. Time
course and ABA treatment assays
showed that promotion of GUS activity
was correlated with higher ABA
concentration and longer treatment time.
In histochemical analysis on T0 and T1
plants, GUS expression was enhanced
by 20μM ABA drastically in roots,
leaves and anthers. Besides, 1%
sodium chloride or chilling could also
increase GUS expression , but were not
as good as ABA treatment.