Nucleic acid detection, quantification and sequence analysis of canine distemper virus from clinical specimens
Date Issued
2005
Date
2005
Author(s)
Huang, Hsuan-Shian
DOI
zh-TW
Abstract
Canine distemper virus (CDV) infection in dogs can result in subclinical infection, gastrointestinal signs, and/or respiratory signs, frequently with central nervous system (CNS) involvement, high morbidity and mortality. Recently, the incidence of canine distemper (CD) both in unvaccinated and vaccinated dogs seemed increasing in Taiwan. In order to understand more about the current CDV infection in Taiwan, a rapid and sensitive diagnotic test for CD using a RT-PCR combined with nested PCR was applied to 440 dogs clinically suspected with CDV infection. 660 clinical specimens including CSF, whole blood, nasal swab, ocular swab, rectal swab and urine were collected from 440 dogs. The results showed that Nested PCR (37.7%, 166/440) increase 20.7% in the efficiency of the diagnosis than RT-PCR (17.0%, 75/489) (P<0.001). The seasonal distribution of the infection was mainly seen in late autumn to winter (P<0.001). Detection rate of conjunctival scraping (100.0%, 11/11) had a significant elevation compared with whole blood (64.4%, 38/59) (P<0.05). Quantification of the viral RNA concentration in clinical specimens from 27 cases by real-time PCR revealed that the highest concentration was as high as 1.8 x 1010 copies/mL. Different specimens from the same animal could vary up to 109 times. Nasal swab, ocular swab, oral swab and rectal swab had higher viral load, indicating that virus was shed in respiratory tract, digestive tract and urinary tract correlated to their clinical syndrome. Nasal swab was found to have the highest viral copies in cases with or without viremia. Therefore, the blood is not the best choice for clinical diagnosis. The result of H gene sequence analysis showed, that Taiwanese CDV is in the lineage of CDV Asia-1.
Subjects
犬瘟熱病毒
H 基因之序列分析
canine distemper virus
sequence analysis of H gene
SDGs
Type
thesis
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