行政院國家科學委員會專題研究計畫成果報告:綠膿桿菌外毒素A對大鼠巨噬細胞之致害及其作用機制(I)形態學、存活率以及核酸、蛋白質及細胞素產生能力
Date Issued
2000
Date
2000
Author(s)
DOI
892313B002059
Abstract
To study the immunotoxic potential of
Pseudomonas aeruginosa exotoxin A (ETA),
changes in the viability, large molecule synthesis,
morphology, and cytokine production of rat
peritoneal macrophages (RPM) were evaluated in
vitro following incubation with ETA at
concentrations of 0, 1, 10, 50, and 100 ng/ml for
3 to 60 hours. A dose and time-dependent
decrease in the viability was revealed in the
ETA-treated RPM as measured by trypan blue
dye exclusion. When compared at the total level,
a similar trend of dose and time-dependent
decrease was also seen in the MTT metabolism
rate and DNA, RNA, and protein synthesis,
although a transient but significant elevation in
the RNA and/or protein synthesis appeared at 24-
36 hours post-incubation (HPI) with 1-50 ng/ml
ETA. However, when compared at the level of
per viable cell the MTT reduction rate became
enhanced with dose at most of the selected time
periods. Except for the DNA synthesis in 1 and
10 ng/ml ETA-treated groups and the RNA and
protein synthesis in 1 ng/ml ETA-treated group,
there was an apparent enhancement in the DNA,
RNA, and protein synthesis on the basis of per
viable cell, particularly at the dose of 50 ng/ml.
Morphologically, necrosis and occasional
apoptosis occurred in the majority of ETAtreated
RPM at 50 or 100 ng/ml after 15 or 6
HPI, respectively. When incubated with 10 ng/ml
of ETA for 24-48 hours, 40% of the RPM were
dead but the remaining survival cells became
swollen due to formation of variably sized
intracytoplasmic granules. Sequential
transmission electron microscopic studies
Pseudomonas aeruginosa exotoxin A (ETA),
changes in the viability, large molecule synthesis,
morphology, and cytokine production of rat
peritoneal macrophages (RPM) were evaluated in
vitro following incubation with ETA at
concentrations of 0, 1, 10, 50, and 100 ng/ml for
3 to 60 hours. A dose and time-dependent
decrease in the viability was revealed in the
ETA-treated RPM as measured by trypan blue
dye exclusion. When compared at the total level,
a similar trend of dose and time-dependent
decrease was also seen in the MTT metabolism
rate and DNA, RNA, and protein synthesis,
although a transient but significant elevation in
the RNA and/or protein synthesis appeared at 24-
36 hours post-incubation (HPI) with 1-50 ng/ml
ETA. However, when compared at the level of
per viable cell the MTT reduction rate became
enhanced with dose at most of the selected time
periods. Except for the DNA synthesis in 1 and
10 ng/ml ETA-treated groups and the RNA and
protein synthesis in 1 ng/ml ETA-treated group,
there was an apparent enhancement in the DNA,
RNA, and protein synthesis on the basis of per
viable cell, particularly at the dose of 50 ng/ml.
Morphologically, necrosis and occasional
apoptosis occurred in the majority of ETAtreated
RPM at 50 or 100 ng/ml after 15 or 6
HPI, respectively. When incubated with 10 ng/ml
of ETA for 24-48 hours, 40% of the RPM were
dead but the remaining survival cells became
swollen due to formation of variably sized
intracytoplasmic granules. Sequential
transmission electron microscopic studies
Subjects
綠膿桿菌
外毒素A
毒害
腹腔巨
噬細胞
噬細胞
大鼠
Publisher
臺北市:國立臺灣大學獸醫學系暨研究所
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
892313B002059.pdf
Size
54.59 KB
Format
Adobe PDF
Checksum
(MD5):cc36b79fa944669fea0a2bbf46a72b55