Detection of Vibrio anguillarum and Vibrio alginolyticus by randomly cloned DNA fragments
Resource
Taiwania 44 (3): 404-412
Journal
TAIWANIA
Pages
404-412
Date Issued
1999
Date
1999
Author(s)
Hsieh, Y. L.
Tsay, S. S.
Abstract
Vibrio anguillarum and Vibrio alginolyticus were used as tested organisms. Restriction endonuclease HindIII and EcoRI digested DNA fragments from the above two bacteria were randomly selected and inserted into vector pUC19. Among 0.5-2.0 kb DNA fragments were recovered from agarose gel to prepare non-radioactive DIG-labeled probes. One out of 102 cloned fragments could hybridize only to V. alginolyticus. Three out of 94 cloned fragments could hybridize only to V. anguillarum and one fragment is specific for serotype C.
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