Vibrio nitrifigilis sp. nov., a marine nitrogen-fixing bacterium isolated from the lagoon sediment of an islet inside an atoll
Journal
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Journal Volume
114
Journal Issue
7
Pages
933-945
Date Issued
2021
Author(s)
Abstract
A nitrogen-fixing isolate of facultatively anaerobic, marine bacterium, designated strain NFV-1T, was recovered from the lagoon sediment of Dongsha Island, Taiwan. It was a Gram-negative rod which exhibited motility with monotrichous flagellation in broth cultures. The strain required NaCl for growth and grew optimally at about 25–35?°C, 3% NaCl and pH 7–8. It grew aerobically and could achieve anaerobic growth by fermenting d-glucose or other carbohydrates as substrates. NH4Cl could serve as a sole nitrogen source for growth aerobically and anaerobically, whereas growth with N2 as the sole nitrogen source was observed only under anaerobic conditions. Cellular fatty acids were predominated by C16:1ω7c, C16:0, and C18:1ω7c. The major polar lipids consisted of phosphatidylethanolamine and phosphatidylserine. Strain NFV-1T had a DNA G + C content of 42.5?mol%, as evaluated according to the chromosomal DNA sequencing data. Analyses of sequence similarities and phylogeny based on the 16S rRNA genes, together with the housekeeping genes, gyrB, ftsZ, mreB, topA and gapA, indicated that the strain formed a distinct species-level lineage in the genus Vibrio of the family Vibrionaceae. These phylogenetic data and those from genomic and phenotypic characterisations support the establishment of a novel Vibrio species, for which the name Vibrio nitrifigilis sp. nov. (type strain NFV-1T = BCRC 81211T = JCM 33628T) is proposed. ? 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
Subjects
Marine bacteria
Multilocus sequence analysis (MLSA)
Nitrogen-fixing bacteria
Vibrio
Vibrio nitrifigilis
Vibrionaceae
carbohydrate
fatty acid
glucose
nitrogen
phosphatidylethanolamine
phosphatidylserine
sodium chloride
bacterial DNA
phospholipid
RNA 16S
Article
bacterial gene
bacterial strain
bacterium isolation
controlled study
DNA sequencing
ftsZ gene
gapA gene
genetic analysis
gyrB gene
housekeeping gene
lagoon
mreB gene
multilocus sequence typing
nonhuman
sediment
topA gene
bacterium identification
DNA base composition
DNA sequence
genetics
phylogeny
Bacterial Typing Techniques
Base Composition
DNA, Bacterial
Fatty Acids
Nitrogen
Phospholipids
Phylogeny
RNA, Ribosomal, 16S
Sequence Analysis, DNA
SDGs
Publisher
Springer Science and Business Media Deutschland GmbH
Type
journal article