Investigations on Hydrodynamic Focusing by Con-focal Microscopy and Fluorescence Correlation Spectroscopy
Date Issued
2008
Date
2008
Author(s)
Liu, Ching-Chuan
Abstract
In protein folding, early folding events occur on a microsecond to second time scale. In order to study protein kinetics, folding reactions must be triggered in a short time. In this thesis, we design four different micro-mixers based on hydrodynamic focusing. We use con-focal microscopy and fluorescence correlation spectroscopy (FCS) to synthesize qualitatively and quantitatively. Velocity is the most important parameter since it deeply affects the determination of mixing dead time and time resolution of our micro-mixer. Fluorescence correlation spectroscopy is a powerful technique to determine the velocity precisely. We made an overall comparison of four different micro-mixers by FCS and con-focal microscopy.
Subjects
Hydrodynamic focusing
Con-focal microscopy
Fluorescence correlation spectroscopy
Velocity
Protein folding
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-97-R95543056-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):f22f07d7b2f5228d6885494f73171a60