The relationship between fucosylated membrane proteins and cancer metastasis
Date Issued
2012
Date
2012
Author(s)
Pai, Hsin-Yung
Abstract
Lung cancer is a disease and ranked as third among the ten leading causes of human deaths according to statistics reports of the world health organization. In clinical studies, lung cancer of a more malignant state may express more fucosyltransferase IV(Fut IV), which is an enzyme that transfers a fucose to a core N-acetylglucosamine sugar of N-linked glycoprotein via α-1,3/4 linkage. Fucosylation is a common glycosylation process in posttranscriptional modification that can affect signal transduction, cell adhesion and cell-cell recognition. In previous studies, the A549-FutIV cell line was established, which expresses relatively high amounts of Fut IV. A549-FutIV, consists of nineteen membrane proteins with more or less higher degree of fucosylation of their N-glycoproteins when compared with A549-Mock. The functions of most of these membrane proteins remain unknown.
In this study, we focus on the relationship between these membrane proteins and cancer metastasis in A549-FutIV. The protein (SLC3A2, CD166 or CD44) was knockdown by siRNA. CD166 and CD44 expressions correlated with the degree of cancer malignancy. After CD166 and CD44 knockdown, the cancer invasion ability had significantly decreased compared to the control and CD166 could also bind to the extracellular matrix(ECM). SLC3A2 was an amino acid transporter heavy chain that played an important roles as a mediator of fibronectin assembling and also as a biomarker in renal cell cancer. According to our experiments, SLC3A2 did not cause any significantly changed to cancer metastasis, but it could bind to fibronectin. Although CD44 knockdown could inhibit cancer invasion, it did not result from its adhesion ability. By combining invasion assay and adhesion assay results, we found that SLC3A2 knockdown could inhibit cancer invasion through its adhesion ability. However, the inhibition of cancer invasion after CD166 and CD44 knockdown did not occur via their adhesion abilities.
Subjects
Lung cancer
Fucosyltransferase IV
Metastasis
membrane protein
PTM
N-linked glycosylation
invasion assay
SDGs
Type
thesis
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