The study on antioxidation and proteomics of tomato plants infected by Tomato yellow leaf curl Thailand virus
Date Issued
2011
Date
2011
Author(s)
Lin, Yu-Heng
Abstract
Tomato yellow leaf curl disease (TYLCD) induced by Tomato yellow leaf curl virus is one of the most severe diseases of tomato. TYLCD defeats tomato production worldwide and often causes 100％ economic loss. There is no efficient method to control TYLCD, except for breeding resistant cultivars. However, the plant physiology concerning TYLCD infection remains totally unknown. This research studied the antioxidation and proteomics of tomato plants after infection of TYLCD to understand the resistance mechanism. The resistant tomato line CLN2777G-1-5 and susceptible cultivar Tomatoll which were selected and bred by Asian Vegetable Research and Development Center, and Tomato yellow leaf curl Thailand virus (TYLCTHV) were used as experimental materials. The results indicated that TYLCTHV is transmitted by whitefly that not mechanical. The leaf number, plant height, relative chlorophyll content (SPAD value) and chlorophyll fluorescence Fv/Fm of the infected ‘Tomatoll’ plant, were significantly decreased. All plants of susceptible cultivar were detected the occurrence of TYLCTHV DNA and first symptom appeared on 3 and 7 days after inoculation. While eighty three percent plants of resistant line were not detected the occurrence of virus DNA until 28 days and did not show symptom. Activity of superoxide dismutase (SOD) in leaves of resistant line was significantly lower than susceptible cultivar on 1 and 7 days after inoculation. However, the activities of catalase (CAT)、ascorbate peroxidase (APX)、glutathione reductase (GR) and peroxidase of resistant line raised successively after inoculation. The raised activities of antioxidant enzymes may induce the resistance of CLN2777G-1-5 plants to TYLCTHV. Proteomics of leaves analyzed by ProteomeLabTM PF-2D and matrix-assisted laser desorption ionization time-of-flight, thirty proteins with differential expression were identified from CLN2777G-1-5 and‘Tomatoll’after inoculation with TYLCTHV. These proteins involve photosynthesis, stress defense, protein transport, translation, protein lysis, and oxidation-reduction reaction. The results may be helpful to plan strategy and select index for breeding TYLCTHV resistant tomato.
Subjects
Tomato yellow leaf curl Thailand virus
superoxide dismutase
catalase
ascorbate peroxidase
glutathione reductase
peroxidase
proteomics
Type
thesis
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