Rapid protein identification using a disposable on-line clean-up/ concentrating device and electrospray ionization mass spectrometry

Tsai, C. Y.; Pai, P. J.; Ho, Y. H.; Lu, J. Y.; Wang, J. S.; Lin, W. Y.; Her, G. R.; Tsai, C. Y.; Pai, P. J.; Ho, Y. H.; Lu, J. Y.; Wang, J. S.; Lin, W. Y.; Her, G. R.;

doi:10.1002/rcm.2857

Rapid protein identification using a disposable on-line clean-up/ concentrating device and electrospray ionization mass spectrometry

Resource

Rapid Communication for Mass Spectrometry,21(4),459-459.

Journal

Rapid Communications in Mass Spectrometry

Pages

459-465

Date Issued

2007-02

Date

2007-02

Author(s)

Tsai, C. Y.

Pai, P. J.

Ho, Y. H.

Lu, J. Y.

Wang, J. S.

Lin, W. Y.

Her, G. R.

DOI

10.1002/rcm.2857

URI

http://ntur.lib.ntu.edu.tw//handle/246246/204659

Abstract

A simple, low-cost, expedient method has been developed for identification of proteins isolated from two-dimensional (2D) gels. The method described uses a disposable on-line clean-up device, a syringe infusion pump and electrospray ionization mass spectrometry (ESI-MS). The on-line clean-up and concentrating device is a tapered capillary column filled with 1.5 cm of 5 microm C18 particles. The short column was easily prepared and was connected directly to the ESI source through a low-flow ESI sprayer. Peptides resulting from enzymatic digestion of proteins were eluted from the short column isocratically using a syringe infusion pump and analyzed by ESI-MS. This simple set-up was found useful in the analysis of proteins isolated from 2D gels. Compared to the more conventional micro-liquid chromatography/tandem mass spectrometry (microLC/MS/MS), this method can identify proteins rapidly without the need for an HPLC pump and removes the problem of cross-contamination caused by system carryover. These advantages make the method described competitive with conventional LC/MS even though the latter method gives slightly expanded sequence coverage.

SDGs

[SDGs]SDG9

Type

journal article

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Rapid protein identification using a disposable on-line clean-up/ concentrating device and electrospray ionization mass spectrometry

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