Katanin-Dependent Microtubule Ordering in Association with ABA Is Important for Root Hydrotropism
Journal
International Journal of Molecular Sciences
Journal Volume
23
Journal Issue
7
ISSN
16616596
Date Issued
2022-04-01
Author(s)
Abstract
Root hydrotropism refers to root directional growth toward soil moisture. Cortical micro-tubule arrays are essential for determining the growth axis of the elongating cells in plants. However, the role of microtubule reorganization in root hydrotropism remains elusive. Here, we demonstrate that the well-ordered microtubule arrays and the microtubule-severing protein KATANIN (KTN) play important roles in regulating root hydrotropism in Arabidopsis. We found that the root hydrotropic bending of the ktn1 mutant was severely attenuated but not root gravitropism. After hydrostimulation, cortical microtubule arrays in cells of the elongation zone of wild-type (WT) Col-0 roots were reoriented from transverse into an oblique array along the axis of cell elongation, whereas the microtubule arrays in the ktn1 mutant remained in disorder. Moreover, we revealed that abscisic acid (ABA) signaling enhanced the root hydrotropism of WT and partially rescued the oryzalin (a microtubule destabilizer) alterative root hydrotropism of WT but not ktn1 mutants. These results suggest that katanin-dependent microtubule ordering is required for root hydrotropism, which might work downstream of ABA signaling pathways for plant roots to search for water. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
abscisic acid
cortical microtubule arrays
KATANIN
oryzalin
root hydrotropism
SDGs
Publisher
MDPI
Description
Article Number: 3846
Type
journal article