The regeneration in floral organs culture of bromeliad Neoregelia cv. Perfecta Tricolor and Guzmania cv. Hercules.
Date Issued
2009
Date
2009
Author(s)
Hsu, Yuan-Chu
Abstract
The ovary explants of Guzmania cv. Hercules were inoculated on 1/2 MS medium supplemented with 2,4-D 0.5-2.0 mg/L combined NAA 0.51.0 mg/L , the combination of 2,4-D 1.0 mg/L and NAA 0.5 mg/L was capable to induce yellow granular calli , the frequency was 22.2% . The calli were massively proliferated by serial subcultures. The callus mass were transferred on 1/2 MS medium supplemented with TDZ 1.0 mg/L+NAA 1.0 mg/L, for induction of adventitious buds , about 3-6 buds were formed from each 0.5 cm callus by 2 months after subculture . In further, the ovary-derived adventitious buds were subdivided into 0.5 cm bud blocks , then transferred to proliferation medium as above , each bud block was proliferated 1-5 shoots, the average multiplication rate was 3.2. The multiple buds were separated and transferred to cytokinin free medium for shoot elongation . The singular bud was isolated and rooted rosette plantlets were achieved on medium containing NAA 0.5 mg/L by 2 months after microculture.he ovary explants of Guzmania cv. Carine were inoculated on 1/2 MS medium supplemented with 2,4-D 1.0 mg/L、NAA 1.0 mg/L, yellow calli were induced , the frequency was 25%, and those calli were propagated massively by subculture.he petal explants of Neoregelia cv. Perfecta Tricolor were inoculated on 1/2MS medium added with NAA 0.5 mg/L combined with 2,4-D 1.0 mg/L、1.5 mg/L、2.0 mg/L、1.5 mg/L+BA 0.5 mg/L , the callusing frequency were 91.6%、83.3%、66.6%、58.3% , white calli were induced , and the treatment of 2,4-D 1.0 mg/L was better . The other floral organs included sepal、stamen、ovary and stigma explants were inoculated on 1/2 MS + 2,4-D 1.0 mg/L+NAA 0.5 mg/L, obtained callusing frequency were 25%、91.6%、75% and 75% respectively . But the problem of browning still needs to be resolved. Besides, when supplemented with 2,4-D 1.5 mg/L+BA 0.5 mg/L+NAA 0.5 mg/L , the translucent friable callus and white granular callus could be formation at the same time from the floral organ.
Subjects
bromeliad
tissue culture
Type
thesis
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