Characterization of the Ipomoelin promoter from sweet potato
Date Issued
2007
Date
2007
Author(s)
Hsiao, An-Shan
DOI
zh-TW
Abstract
Ipomoelin (IPO), a wound-inducible defense protein from sweet potato (Ipomoea batatas cv. Tainung 57), can be induced by wounding, Methyl Jasmonate (MeJA), and ethylene, and is an excellent material to study plant defense system. The full length of IPO promoter, fragment A (position -1240~-1), and its serial deletion fragments B (-1136~-1 ), C (-836~-1 )、D (-496~-1 ), and E (-239~-1 ) were isolated and constructed by Yu-Lin Li, and their tansient expression in tobacco protoplast has been proceeded. Furthermore, motif deletion fragment F, which has deletion of a ethylene response element (ERE, -1107~-1100 ) between fragments B and C and motif deletion fragment G, of which the length is 1235 bp and has deletion of TGACG motif between fragments D and E (-496~-239), were also analyzed.
With attachment to the b-glucunonidase(GUS) gene, the full length, serial deletion, and motif deletion promoters were transformed into tobacco, and their expressions stimulated by ethylene and MeJA were studied in the stable transformants. Both the experiments of tobacco transient expression and stable transformation proved the effects of both MeJa and ethylene upon the induction of IPO promoter. Also, ERE that is located between -1107~-1100 was responsible for the induction of ethylene, and TGACG motif positioned -496~-239 was involved in MeJA stimulation in IPO promoter. Also, the induction magnification of MeJA upon IPO promoter was higher than that of ethylene, and the induction response of MeJA to IPO promoter was faster than that of ethylene. Interestingly, TGACG-motif functioned as a negative cis-element repressing the activity of IPO promoter in the absence of MeJA, but upon the addition of MeJA TGACG-motif positively helped IPO promoter expression.
Subjects
啟動子
promoter
Type
other
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