Development of optical and electrical biotech methodology: An optically multi-functional biochip system and innovative electro-chemistry methodology
Date Issued
2004
Date
2004
Author(s)
Hsu, Wei-Liang
DOI
zh-TW
Abstract
In this dissertation, a multi-functional optical biochip system was built. This biosensor is developed by integrating ellipsometry and SPR detection techniques, which adopted a paraboloidal mirror and a spherical mirror to vary the incident angle, a LCD phase modulator to control incident light beam retardance, a reference optical path to calculate the phase modulated by LCD, and a flow-injection system to supply bio-samples to biochip. The other important function of this biosensor is to observe SPR phenomenon by using the ellipsometric configuration. By using this method, the SPR resolution could be significantly enhanced, and the characteristics of ellipsometry give detailed information for bio-tech analysis. Information such as film thickness, physical parameters, concentration, mass, density, kinetic constants, binding specificity, etc. can all be retrieved. This biosensor was named OBMorph, which is an acronym of Opto-BioMorphin. In summary, OBMorph could serve both as a research and development tool as well as a manufacturing tool in biomedical area.
In order to achieve high precision goal, we adopt the mirrors and a 0.4μm accuracy servomotor to control incident angle between 20° to 80° within ±0.001° angular accuracy. By identifying Brewster’s angle and SPR angle, the interrogation angle could be retrieved accurately which leads to much higher sensitivity and stability. Moreover, calibration of reference optical path would be carried out, and the phase modulated by LCD would be detected more precisely to make our measurement more reliable. In order to increase signal to noise ratio, the object beam is designed to be reflected by sample twice times, and the extra ellipsometric parameters induced by the system would be detected and eliminated. Eventually, experimental results show that OBMorph, which is integrated with high efficiency flow injection system, could successfully measure not only the immobilized monolayer of IgG and bio-linker but also the thickness of biochip coating.
A novel electro-chemistry method is another issue discussed in this dissertation. There are four methods were examined and all these methods derived their basic thoughts from adopting and modulating sinusoidal waves. From the experimental results, the first two methods are verified through enzymatic methods. With an attempt to expand the applications of OBMorph, this biosensor is also tested to pursue glucose concentration measurement as well.
Subjects
表面電漿共振
電化學檢測
橢圓偏光儀
Ellipsometry
electro-chemistry
Surface plasmon resonance
Type
thesis
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