Quantum-dot-embedded silica nanotubes as nanoprobes for simple and sensitive DNA detection
Resource
NANOTECHNOLOGY, 22(15), 155102
Journal
Nanotechnology
Journal Volume
22
Journal Issue
15
Pages
155102
Date Issued
2011
Date
2011
Author(s)
Liu, Yi-Hsin
Tsai, Yi-Yun
Chien, Hsiao-Ju
Chen, Chien-Ying
Huang, Yu-Feng
Chen, Jinn-Shiun
Chen, Chia-Chun
Abstract
We have developed a new technique using fluorescent silica nanotubes for simple and sensitive DNA detection. The quantum-dot-embedded silica nanotubes (QD-SNTs) were fabricated by a sol-gel reaction using anodic aluminum silica oxide (AAO) as a template. The fluorescent QD-SNTs of different colors were then immobilized with single-stranded DNA and used as nanoprobes for DNA detection. The optical and structural properties of QD-SNT nanoprobes were examined using photoluminescence spectroscopy, confocal microscopy and transmission electron microscopy (TEM). The QD-SNT nanoprobes were applied to detect dye-labeled target DNA in a solution phase. The obvious color change of the QD-SNT nanoprobes was observed visually under a simple microscope after the successful detection with target DNA. The quantitative analyses indicated that ∼ 100 attomole of target DNA in one nanoprobe can generate a distinguishable and observable color change. The detection results also demonstrated that our assay exhibited high specificity, high selectivity and very low nonspecific adsorption. Our simple DNA assay based on QD-SNT nanoprobes is expected to be quite useful for the needs of fast DNA screening and detection applications. © 2011 IOP Publishing Ltd.
Other Subjects
Attomole; Color changes; DNA assays; DNA detection; DNA screening; High selectivity; High specificity; Nonspecific adsorption; Quantitative analysis; Quantum dots; Silica nanotubes; Silica oxide; Single-stranded DNA; Sol-Gel Reaction; Solution phase; TEM; Adsorption; Anodic oxidation; Color; Colorimetry; Confocal microscopy; DNA; Fluorescence; Nanoprobes; Nanotubes; Photoluminescence spectroscopy; Semiconductor quantum dots; Silica; Transmission electron microscopy; Nucleic acids; 5 carboxytetramethylrhodamine succinimidyl ester; 5-carboxytetramethylrhodamine succinimidyl ester; carbocyanine; complementary DNA; cyanine dye 3; DNA; fluorescein isothiocyanate; fluorescent dye; nanotube; quantum dot; rhodamine; silicon dioxide; adsorption; article; chemistry; DNA probe; fluorescence microscopy; nucleic acid hybridization; particle size; spectrofluorometry; transmission electron microscopy; Adsorption; Carbocyanines; DNA; DNA Probes; DNA, Complementary; Fluorescein-5-isothiocyanate; Fluorescent Dyes; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Nanotubes; Nucleic Acid Hybridization; Particle Size; Quantum Dots; Rhodamines; Silicon Dioxide; Spectrometry, Fluorescence
Type
journal article
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