The Relationship between Beta 2-Microglobulin (B2m) and the Reproductive Performance of the Landrace Sow (Sus scrofa)
Date Issued
2012
Date
2012
Author(s)
Yang, Ji-Ren
Abstract
During pregnancy, the immune system of female mammals tolerates a non-self antigen-presenting embryo. Therefore, a fine-tuned mechanism of antigen presenting between the maternal-fetal interface during implantation is crucial for embryo survival. Antigen presenting requires MHC (major histocompatibility complex) molecules on the cell surface, and one of the components beta 2-microglobulin, is prerequisite for the complex to be located on cell surface. In this study, monoclonal antibody against B2m was used to evaluate the reproductive performance of Landrace sows and its relationship between serum B2m. In two-dimensional Western blot analyses, serum B2m from both high-productive and low-productive premating sows showed several spots around 28 kDa with different pI, which may result from posttranslational modification. Comparing the protein levels between these spots, we found that high-productive sows showed higher protein intensity for the two acidic B2m spots, but not in low-productive sows. Based on this pattern, prediction of productivity performance experiments were conducted to predict the productivity of premating landrace sows. Four batches of serum samples were collected in different mating seasons. The first two predictions of accuracy in cool season were both 100%, and the one in warm season was 90%. However, the one in hot season only reached predicted 50% accuracy. Thus, the seasonal temperature changes may influence the physiological state of pregnant sows, and further affecting the prediction accuracy by serum B2m pattern. In conclusion, the modification of B2m leading to different spots and the seasonal effects need further study to elucidate the relationship between B2m and reproductive performance of sows.
Subjects
Landrace sow
Implantation
Beta 2-microglobulin (B2m)
Reproductive performance
Two-dimensional electrophoresis
Western blot analysis
Type
thesis
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