Coculture of Endothelial and Smooth Muscle Cells on a Collagen Membrane in the Development of a Small-Diameter Vascular Graft
Resource
BIOMATERIALS v.28 n.7 pp.1385-1392
Journal
BIOMATERIALS
Journal Volume
v.28
Journal Issue
n.7
Pages
1385-1392
Date Issued
2007
Date
2007
Author(s)
WU, HSI-CHIN
WANG, TZU-WEI
KANG, PEI- LEUN
TSUANG, YANG-HWEI
SUN, JUI-SHENG
LIN, FENG-HUEI
Abstract
In this study, we have evaluated the feasibility of developing a biodegradable collagenous small diameter vascular graft of 2mm diameter and 1 cm length. In brief, bi -layer type I collagen membrane was fabricated under vacuum suction and lyophilization methods. The smooth muscle cells were inoculated into the lower side of the porous membrane, while endothelial cells were seeded onto upper smooth side of the membrane . After cultured for 7 days, the vascular substitute was either harvested for in vitro examination or in vivo implanted in the subcutaneous layer for biocompatibility test. The tubular vascular prosthesis was then used as a temporary absorbable guide that served as an in vivo vascular graft to promote the complete regeneration of rat inferior vena cava. After implantation for 12 weeks, a thin continuous layer of endothelial cells and smooth muscle cells were lined with the vascular lumen and tunic media , respectively. Histology results showed that there were no signs of significant thrombogeneity and intima hyperplasia. This tissue engineered vascular substitute not only had enough tensile strength and good biocompatibility, but also advanced vascular regeneration. In the future, we suggest that this biodegradable vascular substitute will provide with the possibility in application on small diameter prosthetic grafts in artificial blood vessels. (c) 2006 Elsevier Ltd. All rights reserved.
Subjects
biomimetic material
collagen
co-culture
vascular grafts