Porcine Circovirus Type 2 effects on Peripheral Blood ononuclear Cells by Concanavalin A Stimulation
Date Issued
2009
Date
2009
Author(s)
Hsu, Ching-Lan
Abstract
Porcine circovirus type 2 (PCV2) is associated with a new emerging disease, ostweaning multisystemic wasting syndrome (PMWS) which causes severe economic osses in swine industry worldwild. PMWS-affected pig is characterized by high PCV2 oad in serum, ranging from 3.9×105 to 1.8×1012 PCV2 copies/mL, which has ignificantly correlated with severe clinical signs of PMWS. Several reports reveal mmune stimulation is an important factor in the induction of PMWS. Con A enhances CV2 replication and high viral titer in PBMC and in lymphoid tissues, respectively, hat has correlated with PMWS as well. The aim of this study is to investigate the mpact of PCV2 infection on Con A stimulated cell proliferative response. The xperimental designs include: 1. To evaluate the correlation between viral load in serum nd Con A stimulated cell proliferation as well as IFN-γ and IL-10 secretions in PBMC rom PCV2-carrier pigs. 2. To evaluate whether PCV2 superinfection affect Con A timulated cell proliferative response in experimental PCV2 infected SPF pigs. Flow ytometry was used to detect cell proliferation response and ELISA was used to valuate the level of IL-10 and IFN-γ. Viral load was measured by TagMan® Real-time CR. Culture of PBMC or spleen cells were separated into 4 group of treatment as ock, PCV2 superinfection, Con A treated and Con A/PCV2. In the first part of the tudy, the tendency of inverse correlation between seral PCV2 load and proliferation esponse of PBMC at Con A/PCV2 group was observed. The postitive correlation etween IL-10 level and viral load was also noted. In the second part of the study, BMC proliferation after Con A stimulation could be enhanced by PCV2 superinfection n the control group. However, this enhancement was not observed in the experimental CV2 infected SPF pigs. In this group, the inhibition of enhanced cell proliferation was orrelated to the cellular viral load but was not associated with the process of necrosis r apoptosis and might be associated with high IL-10 level. In spleen, viral load in pleen cells and IL-10 secretions were insignificant but the level of IFN-γ was enhanced t Con A/ PCV2 group. Our data suggested that high viral load could inibit Con A timulated cell proliferation and the inhibition maybe associated with induction of IL-10.
Subjects
IL-10
PBMC
lymphocyte proliferative response
Type
thesis
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