Electrophoretic behavior of cerebellar granule neurons
Journal
Electrophoresis
Journal Volume
23
Journal Issue
13
Pages
2001-2006
Date Issued
2002
Author(s)
Abstract
The electrophoresis of cerebellar granule neurons is observed, and a theoretical model proposed to simulate its electrophoretic behavior. We assume that the surface of a neuronal cell carries dissociable acidic functional groups, and the liquid phase contains a mixed (a:b) + (c:b) electrolyte, where a and c are the valences of cations and b is the valence of anions. The cations of valence c are allowed to bind to dissociated functional groups. The model proposed is readily applicable to the prediction of the surface properties of cerebellar granule neurons such as the density of dissociable functional groups and the equilibrium constant of the dissociation reaction. The applicability of the present model is justified by fitting it to the measured electrophoretic mobility data.
Subjects
Cerebellar granule neurons; Electrophoretic mobility; Ion-binding; Surface dissociation reaction; Surface properties
Other Subjects
acid; anion; cation; electrolyte; functional group; animal cell; article; binding affinity; brain nerve cell; cell surface; cerebellum; controlled study; data analysis; density; dissociation; electrophoresis; equilibrium constant; liquid; nonhuman; prediction; rat; theoretical model; animal; cell motion; cell separation; cytology; electrochemistry; methodology; nerve cell; Wistar rat; Animal; Cell Movement; Cell Separation; Cerebellum; Electrochemistry; Electrophoresis; Neurons; Rats; Rats, Wistar; Support, Non-U.S. Gov't; Animals; Cell Movement; Cell Separation; Electrochemistry; Rats; Rats, Wistar
Type
journal article