Karyotyping and localization of functional genes on chromosomes of Ganoderma lucidum
Date Issued
2010
Date
2010
Author(s)
Lin, Wei-Chun
Abstract
In nature, Ganoderma licidum acts as wood white-rot pathogens for many tree species; nevertheless, its fruit body has also been used for century as folk medicine. More recently, researches have shown its polysaccharides or terpenoides with anti-tumor, alleviating high blood pressure and hepatoprotective activity. Though immensive biological activity studies of G. lucidum have been focused on its secondary metabolites, the basic exploration appeared rare. Herein, we concentrated our efforts to study the chromosomal and karyotyping of G. lucidum via germ tube bursting method (GTBM), pulse field gel electrophoresis (PFGE), fluorescence in situ hybridization (FISH), flow cytometry and scanning electromicroscopy. GTBM in combination of fluorescent microscopy indicated that G. lucidum 37180 (A2B2) possessed 11-15 chromosomes, with average of 12.65, and a length of 0.8~4.1 µm, while PFGE showed the six-banded chromosomal zones of 3.02, 3.36, 3.67, 4.24, 4.53 and 5.05 Mb. One additional chromosomal band at 4.0 Mb was found in G. lucidum 37177 (A1B1). Southern blotting of the six GL 37180 chromosomes bands separated by PFGE by using specific probes derived from the thirteen deciphed GL 37177 chromosomes unraveled the 13 allelic chromosomes, respectively. In addition, by specific probe (A2: b1 gene) also can allocate GL A2 mating locus located at chromosome I, also allocating the possible location of B2 mating locus possibly at chromosome III, V or VI by specific probe (B2: PR8 gene). The same approaches undertaken in this study perhaps can be applied to study the karyotyping of other GL monokaryotic strains and other medicinal mushrooms as well.
Subjects
Ganoderma lucidum
chromosome
karyotyping
germ tube burst method
pulsed field gel electrophore
Type
thesis
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