Mutation and lineage analysis of DNMT3A in BCR-ABL1-negative chronic myeloproliferative neoplasms
Journal
International Journal of Gerontology
Journal Volume
7
Journal Issue
3
Pages
186-188
Date Issued
2013
Author(s)
Lin, Huan-Chau
Wang, Shu-Ching
Chen, Caleb Gon-Shen
Chang, Ming-Chih
Wang, Wei-Ting
Su, Nai-Wen
Cheng, Hung-I
Lin, Johnson
Chang, Yi-Fang
Hsieh, Ruey-Kuen
Chang, Chien-Chung
Hwang, Yuchi
Lim, Ken-Hong
Abstract
In addition to the JAK2 V617F mutation, somatic mutation in DNMT3A has been described in BCL-ABL1-negative myeloproliferative neoplasms (MPNs). We have screened for DNMT3A exon 23 mutations in 130 adult Taiwanese patients with chronic phase myeloproliferative neoplasms. Only one somatic DNMT3A R882H mutation was identified in one JAK2 V617F mutation-positive essential thrombocythemia patient (1/91, 1%). Both mutations were detected in the CD34+-, CD19+-, peripheral blood mononuclear cell- and granulocyte-enriched fractions, but were not detected in the CD3 +-enriched fraction by lineage analysis. Our findings suggest that DNMT3A mutation is not prevalent in MPNs, and further study is needed to clarify its role in the molecular pathogenesis of myeloproliferative neoplasms. ? 2013, Taiwan Society of Geriatric Emergency & Critical Care Medicine. Published by Elsevier Taiwan LLC. All rights reserved.
Subjects
DNMT3A; mutation; myeloproliferative neoplasm
SDGs
Other Subjects
BCR ABL protein; CD19 antigen; CD34 antigen; DNA methyltransferase 3A; article; cell fractionation; exon; granulocyte; human; major clinical study; molecular pathology; myeloproliferative neoplasm; nucleotide sequence; peripheral blood mononuclear cell; priority journal; somatic mutation; Taiwan; thrombocythemia
Type
journal article