Gelatin Nanoparticles as DNA Carriers for Transgenic Chicken Application
Date Issued
2008
Date
2008
Author(s)
Peng, Chu-Li
Abstract
In thus study, we tried to use gelatin as carriers in gene delivery, and prepare the gelatin nanoparticles with particle size of 300nm. For calculating the capturing ratio, the DNA loading efficiency is nearly 80％. Size analysis and stability were performed under dynamic light scattering and TEM. DNA encapsulated in the gelatin nanoparticles could be protected from DNaseΙdegradation. In HeLa cell transfection experiments, efficiencies of commercial product LipofectaineTM 2000 and gelatin/ pEGFP-C1 nanoparticles were nearly the same. When comparing to commercial product LipofectaineTM 2000, gelatin/pEGFP-C1 nanoparticles had higher cell viability and lower cytotoxicity. After gelatin nanoparticles transfection, the egg hatchability was 80-100％. We could also detect Green fluorescence in the fourth day chicken embryo transfected by gelatin/pEGFP-C1 nanoparticles.
Subjects
gelatin nanoparticles, transgenic chickens
Type
thesis
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