Ligand conformation dictates membrane and endosomal trafficking of arginine-glycine-aspartate (RGD)-functionalized mesoporous silica nanoparticles
Resource
Chemistry - A European Journal, 18(25), 7787-7792
Journal
Chemistry - A European Journal
Journal Volume
18
Journal Issue
25
Pages
7787-7792
Date Issued
2012
Author(s)
Abstract
Recent breakthrough research on mesoporous silica nanoparticle (MSN) materials has illustrated their significant potential in biological applications due to their excellent drug delivery and endocytotic behavior. We set out to determine if MSN, covalently functionalized with conformation specific bioactive molecules (either linear or cyclic RGD ligands), behave towards mammalian cells in a similar manner as the free ligands. We discovered that RGD immobilized on the MSN surface did not influence the integrity of the porous matrix and improved the endocytosis efficiency of the MSN materials. Through competition experiments with free RGD ligands, we also discovered a conformation specific receptor-integrin association. The interaction between RGD immobilized on the MSN surface and integrins plays an important role in endosome trafficking, specifically dictating the kinetics of endosomal escape. Thus, covalent functionalization of biomolecules on MSN assists in the design of a system for controlling the interface with cancer cells. ? 2012 Wiley-VCH Verlag GmbH&Co. KGaA, Weinheim.
Subjects
Cell recognition; Endocytosis; Integrin; Mesoporous materials; RGD
SDGs
Other Subjects
Bioactive molecules; Biological applications; Cancer cells; Cell recognition; Covalent functionalizations; Endocytosis; Endosomes; Free ligands; Functionalized; Integrins; Ligand conformations; Mammalian cells; Mesoporous silica nanoparticles; Porous matrixs; RGD; Amino acids; Drug delivery; Mesoporous materials; Molecular biology; Ligands; arginyl-glycyl-aspartic acid; arginylglycylaspartic acid; ligand; nanoparticle; oligopeptide; silicon dioxide; article; chemistry; drug design; drug screening; endocytosis; female; HeLa cell; human; nuclear magnetic resonance; Drug Design; Drug Screening Assays, Antitumor; Endocytosis; Female; HeLa Cells; Humans; Ligands; Nanoparticles; Nuclear Magnetic Resonance, Biomolecular; Oligopeptides; Silicon Dioxide
Type
journal article
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