疫病菌致病分子機制之探討
Date Issued
2004
Date
2004
Author(s)
DOI
922313B002063
Abstract
Phytophthora parasitica Dastur (=Phytophthora nicotianae Breda de Haan), an oomycete
plant pathogen, caused severe diseases in a wide variety of plant species. In eukaryotes, the MAP
kinase pathways are activated in response to a wide variety of extracellular signals, and thereby
regulate the processes of growth, differentiation, and pathogenesis in many organisms. As a first
step to investigate the role of MAP kinase pathway in the pathogenesis of P. parasitica,
full-length cDNA encoding MAP kinase was obtained by reverse transcription-PCR and rapid
amplification of cDNA ends (RACE). A total of 4 genes were identified, designated as ppmk1.1,
ppmk1.2, ppmk2, and ppmk3, respectively. Analysis by genomic Southern hybridization revealed
the existence of a single copy of ppmk1.1, ppmk1.2, and ppmk3, respectively, in the genome of P.
parasitica. However, more than one band were detected with ppmk2, suggesting the presence of
other ppmk2-like sequence. Analysis of the deduced amino acid sequences indicated that the
MAP kinases of P. parasitica were highly conserved with those of fungal pathogens. Possible
roles of each MAP kinase in the physiology, life cycle, and pathogenesis of P. parasitica await
further investigation.
Subjects
Phytophthora parasitica
pathogenesis
Publisher
臺北市：國立臺灣大學植物病理與微生物學系暨研究所
Type
other
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