Immunomodulation of saccharomyces cerevisiae on intranasalsal immunization in BALB/c mice
Date Issued
2009
Date
2009
Author(s)
Wei, Chung-Fan
Abstract
β-glucans as β(1→3), β(1→6) binding polysaccharides derived from fungal cell wall, like Ganoderma lucidum and yeasts. β-glucans have been reported that confers immunomodulation with oral administration or intravenous injection. The immunomodulated functions of β-glucans may promote lymphocytes tumor cytotoxic ability or protect against microbial infections. Nevertheless, some kinds of β-glucan can also skew Th1/Th2 balance, may inhibit allergic response. Intranasal administrated nasal vaccines elicit hosts’ systemic specific immunoglobulin G (IgG), also elicit scretory IgA that may neutralize respiratory pathogens at local mucosa. Nasal vaccines are potential, since they are easily accessible for large population, and required without needles and syringes. In present research, we nasal immunized BALB/c mice with heat-labile enterotoxin(LT) from wild-type entero-pathogenic E. coli and ovalbumin (OVA) for 4 times, 1-week internal. In parallel, we oral administrated the mice with sterile water, heat-killed sacchromyces cerevisiae, or commercial sacchromyces cerevisiae legume fermentated product trice a week, started from 1 week before the first nasal immunization, and last to the second week after the last immunization, mice were sacrificed. Saliva, serum of mice was collected every week since feeding started, the samples were detected the specific anti-OVA IgA, IgG and IgE with ELISA. The present data shows that anti-OVA salivary IgA and anti-OVA serum IgG1, IgG2a and IgE in sacchromyces cerevisiae and commercial sacchromyces cerevisiae legume fermentated product-feeding mice with LT+OVA immunized. It’s preliminarily considered that sacchromyces cerevisiae or related products-intake may enhance specific antibody production, through IL-5 and IFN-γ-secreting increased. Therefore, oral intake sacchromyces cerevisiae or related products may protect hosts against pathogen, enhance nasal vaccine efficacy, but increase IgE-mediated allergy risk.
Subjects
immunomudulation
intranasal immunization
salivary IgA
serum immunoglobulins
SDGs
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