New bioactive clerodane diterpenoids from the roots of Casearia membranacea
Journal
Chemistry and Biodiversity
Journal Volume
5
Journal Issue
1
Pages
162-167
Date Issued
2008
Author(s)
Abstract
Bioassay-guided fractionation of the acetone extract of the roots of Casearia membranacea furnished three new clerodane diterpenes, caseamembrins S-U (1-3) and the known caseamembrin Q (4). Their structures were established by extensive spectroscopic analyses, especially 2D-NMR. Compounds 1-4 were tested against human tumor cells, including HeLa (cervical epitheloid carcinoma), DLD-1 (colon carcinoma), Daoy (medulloblastoma), and KB (oral epidermoid carcinoma) cell lines. Caseamembrin T (2) exhibited the most potent activity against Daoy cells (ED50= 10 ng/ml), superior to that of the standard drug mitomycin. ? 2008 Verlag Helvetica Chimica Acta AG, Z?rich.
SDGs
Other Subjects
acetone; antineoplastic agent; caseamembrin Q; caseamembrin S; caseamembrin T; caseamembrin U; Casearia membranacea extract; clerodane derivative; diterpenoid; mitomycin; plant extract; unclassified drug; antineoplastic activity; article; bioassay; cancer cell culture; carbon nuclear magnetic resonance; Casearia; Casearia membranacea; colon carcinoma; concentration response; controlled study; drug potency; drug structure; HeLa cell; herbal medicine; human; human cell; infrared spectroscopy; medulloblastoma; mouth carcinoma; plant root; proton nuclear magnetic resonance; squamous cell carcinoma; ultraviolet spectroscopy; uterine cervix carcinoma; Antineoplastic Agents; Casearia; Cell Line, Tumor; Cell Proliferation; Diterpenes, Clerodane; Drug Screening Assays, Antitumor; Hela Cells; Humans; Magnetic Resonance Spectroscopy; Molecular Conformation; Plant Roots; Reference Standards; Stereoisomerism; Structure-Activity Relationship; Casearia membranacea
Type
journal article