Identification of Human Novel microRNAs and Their Targets
Date Issued
2007
Date
2007
Author(s)
Lee, Tzu-Ying
Abstract
MicroRNAs are endogenous RNAs of 20-23 nucleotides in length that negatively regulate the expression of target genes by binding to the 3’-untranslated regions (UTRs) of the target mRNAs. The importance of miRNAs in animals is highlighted by the facts that they regulate a large proportion of protein-coding genes, and are involved in the control of a variety of processes including development, cell proliferation, tissue differentiation, apoptosis and metabolism. In addition, microRNAs are shown to be directly involved in cancer initiation and progression. Recent studies suggest that up to one third of human genes are regulated by miRNAs. However, although many human miRNA genes have been identified or predicted, it is believed that many more remain to be discovered. In this work, we identified the existence and measured the endogenous expression levels of novel miRNAs predicted by Dr. Wen-Hsiung Li and Dr. Arthur Chun-Chieh Shih; furthermore, we identified their target gene in different kinds of cell lines such as human breast cancer cell line MCF-7, non-tumorigenic MCF-10A and human lung fibroblasts IMR-90. In addition, we analyzed and verified the functions of the novel miRNAs using their mimics and inhibitors by luciferase reporter assay system and western blotting.
Subjects
microRNA
target gene
tissue-selective
luciferase assay
SDGs
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