Decellularized lymph node scaffolding as a carrier for dendritic cells to induce anti-tumor immunity
Journal
Pharmaceutics
Journal Volume
11
Journal Issue
11
Date Issued
2019
Author(s)
Abstract
In recent decades, the decellularized extracellular matrix (ECM) has shown potential as a promising scaffold for tissue regeneration. In this study, an organic acid decellularized lymph node (dLN) was developed as a carrier for dendritic cells (DCs) to induce antitumor immunity. The dLNs were prepared by formic acid, acetic acid, or citric acid treatment. The results showed highly efficient removal of cell debris from the lymph node and great preservation of ECM architecture and biomolecules. In addition, bone marrow dendritic cells (BMDCs) grown preferably inside the dLN displayed the maturation markers CD80, CD86, and major histocompatibility complex (MHC)-II, and they produced high levels of interleukin (IL)-1β, IL-6, and IL-12 cytokines when stimulated with ovalbumin (OVA) and CpG oligodeoxynucleotides (CPG-ODN). In an animal model, the BMDC-dLN completely rejected the E.G7-OVA tumor. Furthermore, the splenocytes from BMDC-dLN-immunized mice produced more interferon gamma, IL-4, IL-6, and IL-2, and they had a higher proliferation rate than other groups when re-stimulated with OVA. Hence, BMDC-dLN could be a promising DC-based scaffold for in vivo delivery to induce potent antitumor immunity. ? 2019 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Cancer immunotherapy; Cell-based therapy; Decellularized scaffold; Dendritic cell; Lymph node
SDGs
Other Subjects
B7 antigen; CD86 antigen; CpG oligodeoxynucleotide; gamma interferon; glycoprotein p 15095; interleukin 12; interleukin 1beta; interleukin 2; interleukin 4; interleukin 6; major histocompatibility antigen class 2; ovalbumin; animal cell; Article; bone marrow derived dendritic cell; cell growth; cell population; cell proliferation; controlled study; ex vivo study; extracellular matrix; flow cytometry; immunization; in vivo study; limit of quantitation; lymph node; mouse; nonhuman; scanning electron microscopy; spleen cell; tumor volume
Type
journal article