A MICROFLUIDIC ANTIBIOTIC CONCENTRATION GRADIENT GENERATOR INTEGRATING SURFACE-ENHANCED RAMAN SPECTROSCOPY FOR MULTIPARALLEL ANTIMICROBIAL SUSCEPTIBILITY TESTING
Journal
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
ISBN
9781733419031
Date Issued
2021-01-01
Author(s)
Abstract
We developed a microfluidic device with 64 side branch channels and microwell array integrating surface-enhanced Raman spectroscopy (SERS) sensing to perform multiparallel antimicrobial susceptibility testing (AST). The device can generate a stable antibiotic concentration gradient in each side channel based on diffusion behavior in laminar flow. The microwell array underneath each side channel can perform an efficient and uniform bacteria encapsulation and isolation. By replacing the top microchannel by the SERS substrate, an in situ and high throughput SERS measurement was done to enable a multiparallel AST. Our preliminary result was using Ampicillin to treat susceptible E. coli as a proof-of-concept test.
Subjects
antimicrobial susceptibility testing (AST) | microfluidics | surface-enhanced Raman spectroscopy (SERS)
Type
conference paper