Study on the Effect of Mushroom β-glucan Against Liver Regeneration in Model Fish Tilapia (Oreochromis niloticus)
Date Issued
2014
Date
2014
Author(s)
Hsiao, Chien-Mei
Abstract
The present study aims to examine the effects of mushroom β-glucan in vitro and in vivo on the liver regeneration using tilapia (Oreochromis niloticus) following partial hepatectomy. The results showed that when tilapia liver were treated with mushroom β-glucan the hepatocytes were significantly enhanced in terms of cellular survival and cell proliferation experiment in vitro. The results of in vivo test using the tilapia partial hepatectomy model showed that mushroom β-glucan may significantly induce liver compensatory growth. The relative gene expression for liver regeneration was also enhanced. Immunochemistry observation showed that when the experimental fish was treated with mushroom β-glucan the number of hepatocyte significantly increased, while the gene expression of the cytokines and growth factor were also significantly enhanced. It is therefore suggested that mushroom β-glucan is capable to regulate the immune response and cellular repair factors for the liver.
Subjects
mushroom β-glucan
liver regeneration
cytokine
growth factor
gene expression
Type
thesis