Photoacoustic imaging of multiple targets using gold nanorods
Journal
IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control
Journal Volume
54
Journal Issue
8
Pages
1642-1647
Date Issued
2007-08
Author(s)
Abstract
Photoacoustlc (PA) Imaging has been used mainly for anatomical and functional imaging. Although functionalized nanoparticles also have been developed for PA molecular Imaging, only single targeting has been demonstrated. In this study, PA Imaging of multiple targets using gold nanorods is demonstrated experimentally using HER2 and CXCR4 as target molecules. The two corresponding monoclonal antibodies were conjugated to two types of gold nanorod with different aspect ratios. Gold nanorods with mean aspect ratios of 5.9 and 3.7 exhibited peak optical absorptions at 1000 and 785 nm, respectively. Appropriate selection of laser irradiation wavelength enhances PA signals by 7-12 dB and allows signals from gold nanorods corresponding to specific bindings to be distinguished. This approach potentially allows the expression levels of different oncogenes of cancer cells to be revealed simultaneously. ? 2007 IEEE.
SDGs
Other Subjects
Acoustic imaging; Aspect ratio; Gold; Nanorods; Photoacoustic effect; Functional imaging; Molecular Imaging; Oncogenes; Photoacoustlc (PA) Imaging; Image analysis; contrast medium; gold; nanotube; animal; bladder tumor; cell culture; chemistry; echography; human; image enhancement; instrumentation; laser Doppler flowmetry; letter; liver tumor; methodology; mouse; Western blotting; Animals; Blotting, Western; Contrast Media; Gold; Humans; Image Enhancement; Laser-Doppler Flowmetry; Liver Neoplasms; Mice; Nanotubes; Tumor Cells, Cultured; Urinary Bladder Neoplasms
Type
journal article