Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis
Journal
Journal of Cellular and Molecular Medicine
Journal Volume
24
Journal Issue
17
Pages
9737-9751
ISBN
32672400
Date Issued
2020
Author(s)
Huang H.-Y.
Chou H.-C.
Law C.-H.
Chang W.-T.
Wen T.-N.
Liao E.-C.
Lin M.-W.
Lin L.-H.
Wei Y.-S.
Tsai Y.-T.
Chen H.-Y.
Tan K.-T.
Chang S.-J.
Lee Y.-R.
Chan H.-L.
Abstract
Cancer metastasis is a common cause of failure in cancer therapy. However, over 60% of oral cancer patients present with advanced stage disease, and the five-year survival rates of these patients decrease from 72.6% to 20% as the stage becomes more advanced. In order to manage oral cancer, identification of metastasis biomarker and mechanism is critical. In this study, we use a pair of oral squamous cell carcinoma lines, OC3, and invasive OC3-I5 as a model system to examine invasive mechanism and to identify potential therapeutic targets. We used two-dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) to examine the global protein expression changes between OC3 and invasive OC3-I5. A proteomic study reveals that invasive properties alter the expression of 101 proteins in OC3-I5 cells comparing to OC3 cells. Further studies have used RNA interference technique to monitor the influence of progesterone receptor membrane component 1 (PGRMC1) protein in invasion and evaluate their potency in regulating invasion and the mechanism it involved. The results demonstrated that expression of epithelial-mesenchymal transition (EMT) markers including Twist, p-Src, Snail1, SIP1, JAM-A, vimentin and vinculin was increased in OC3-I5 compared to OC3 cells, whereas E-cadherin expression was decreased in the OC3-I5 cells. Moreover, in mouse model, PGRMC1 is shown to affect not only migration and invasion but also metastasis in vivo. Taken together, the proteomic approach allows us to identify numerous proteins, including PGRMC1, involved in invasion mechanism. Our results provide useful diagnostic markers and therapeutic candidates for the treatment of oral cancer invasion. ? 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd
Subjects
metastasis; oral cancer; progesterone receptor membrane component 1; proteomics
SDGs
Other Subjects
biological marker; cadherin; CD63 antigen; collagenase 3; cyclin A2; cyclin D; cyclin D2; cyclin E; galectin 3; heat shock protein 70; horseradish peroxidase; immunoglobulin antibody; isothiocyanic acid; junctional adhesion molecule a protein; lipocortin 1; lipofectamine; mitogen activated protein kinase p38; penicillin derivative; polycystin 1; progesterone receptor membrane component 1; protein; protein p53; reactive oxygen metabolite; rompun 60; Smad protein; streptomycin; stromelysin; superoxide dismutase; tiletamine plus zolazepam; transcription factor Snail; Twist related protein 1; unclassified drug; uvomorulin; vasculotropin receptor 1; vimentin; xylazine; adult; angiogenesis; animal experiment; animal model; Article; cell cycle arrest; cell invasion; cell migration; cell proliferation; cell viability; chemoluminescence; controlled study; enzyme linked immunosorbent assay; epithelial mesenchymal transition; extracellular matrix; female; flow cytometry; gel electrophoresis; genetic transcription; glycolysis; human; human cell; immunofluorescence; matrix-assisted laser desorption-ionization mass spectrometry; metastasis; mouse; mouth cancer; MTT assay; nonhuman; oxidative stress; protein expression; protein phosphorylation; tumor growth; wound healing assay
Publisher
Blackwell Publishing Inc.
Type
journal article