Osteoblastic activity of human embryonic palatal mesenchymal cells cultured on different inorganic-polycaprolactone composites
Date Issued
2008
Date
2008
Author(s)
Pao, Yu-Lun
Abstract
There are los of artificial bone grafts. It was proved in many experiments that combined type of high polymer and bioceramic bone grafts have more benefits and plasticities. The biocompatible characters of the polycaprolactone were proved in many animal experiments. And it was applicated frequently in drug-released carriers with low cost and using convenient. Based on the dynamic-balancing of bone apatite, we tried to rule out the bone healing effects of different phase of apatites and Calcium Sulfate, for find the good composite bone graft materials. The material characters of different kinds of Polycaprolactone composited bioceramics including Hydroxyapatite、Calcium Diphosphate、 Calcium phosphate dibasic and Calcium Sulfate were compared in this examination . After many trial and error, we figured out that good porous scaffolds could be fabricated by Freeze-dried method with 1,4 Dioxan as solvent. The human embryonic palatal mesenchymal cells was selected as cell mode for observing the osteo-induction 、 osteo-conduction effects and the serial expressions of bone related genes including ALP 、OC 、BMP2.We found that the cell adherence of our all materials can reach the level of the control dishes. We found that the cell adherence of our all materials can reach the level of the control dishes. And the high ALP expression was noted 24 days after cell seeding. But the CT scan bone densities in animal experiments of our all composite materials were not so good to our expectation. We suppose that the absorption rates of the materials were slower than the bony formation, and the degradable small polymer particle may cause enhanced inflammation effects.
Subjects
HEPM
PCL
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