Disintegration of aerobic granules: Role of second messenger cyclic di-GMP
Journal
Bioresource Technology
Journal Volume
146
Pages
330-335
Date Issued
2013
Author(s)
Abstract
Loss of structural stability of aerobic granular process is the challenge for its field applications to treat wastewaters. The second messenger, cyclic diguanylate (c-di-GMP), is widely used by bacteria to regulate the synthesis of exopolysaccharide. This study for the first time confirmed the correlation between concentration of intracellular c-di-GMP and the granular stability under sequencing batch reactor (MBR) mode. In the presence of manganese ions (Mn2+), the concentrations of intracellular c-di-GMP and of extracellular polysaccharides and proteins in granules were declined. Clone library study revealed that the polysaccharide producers. Acinetobacter sp., Thauera sp., Bdellovibrio sp. and Paracoccus sp. were lost after Mn2+ addition. The findings reported herein confirmed that the c-di-GMP is a key chemical factor epistatic to quorum sensing to determine granular stability. Stimulation of synthesis of intracellular c-di-GMP presents a potential way to enhance long-term stability of aerobic granules. ? 2013 Elsevier Ltd.
Subjects
Aerobic granule
Clone libraries
Cyclic di-GMP
Disintegration
Polysaccharide
SDGs
Type
journal article