Comparison of Immunohistochemistry, Polymerase Chain Reaction, and Loop-Mediated Isothermal Amplification for the Diagnosis of Swine Central Nervous System Diseases from Archival Paraffin Sections
Date Issued
2008
Date
2008
Author(s)
Chen, Yu-Ling
Abstract
Based on the previous studies, histological examination has a limitation to make a definite diagnosis of pathogens of central nervous diseases (CNS). To develop convenient diagnostic tools with high potential for rapid and specific diagnosis for this purpose, non-biotin horseradish peroxidase (non-biotin HRP) immunohistochemistry, loop-mediated isothermal amplification (LAMP), which amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions, and polymerase chain reaction (PCR) are employed in this study. A total of 84 pig cases with miscellaneous CNS diseases were obtained from archival paraffin sections which have been stored up to 20 years. In non-suppurative meningoencephalitis, including 23 pigs with hog cholera, 14 pigs with pseudorabies, 5 pigs with Salmonella sp. infection, 5 pigs with Toxoplasma gondii infection, 13 pigs with unidentified diseases. In suppurative meningoencephalitis, including 24 pigs with Streptococcus suis type 2 meningitis. All cases with any of 5 pathogenic infections were successfully diagnosed by non-biotin HRP immunohistochemistry. DNA from 26 suspected pseudorabies specimens were successful extracted. Among them, 4 samples were amplified and the target nucleic acid was detected by PCR, and 8 samples were amplified and the target nucleic acid was detected by LAMP. The preliminary results demonstrate that non-biotin HRP immunohistochemistry, LAMP, and PCR possess varying potential to detect pathogens of swine CNS diseases for infectious and retrospective studies.
Subjects
Central nervous diseases
Non-biotin horseradish peroxidase
Immunohistochemistry
Loop-mediated isothermal amplification
Archival paraffin sections
SDGs
Type
thesis
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